Abstract:
Azithromycin IS a semi-synthetic macrolide antibiotic chemically related to
erythromycin and clarithromycin. It is used to treat certain infections caused by
bacteria, such as bronchitis, pneumonia, sexually transmitted diseases (STD) and
infections of the ears, lungs, skin, and throat. Quality of drugs must be controlled to
get desired therapeutic effect. In a pharmaceutical sense, quality means checking and
directing the degree or grade of excellence of processes and products. Antimicrobial
susceptibility test is one of the quality control tests to estimate the quality and purity
of antibiotics. It is performed by using disk diffusion test. The objective of the thesis
is to measure the sensitivity and purity of the antibiotic by comparing with the
standard. It is significant to determine the quality and purity of antibiotics because of
rapid occurrence of antibiotic resistance due to use of substandard or poor quality
drugs. The test was performed using Tridosil and Azimex-500 in three different
concentrations such as 50 n;g, 75 n;g and 100 n;g. Three different species of bacteria
such as E. coli, Staphylococcus aureus, Salmonella typhi were inoculated on nutrient
agar plates and different concentrations of sample were applied by disc diffusion
method and incubated overnight. Inhibitory activity of the antibiotic was measured as
zone of inhibition in mm. The zone of inhibition of the sample was compared with the
zone of inhibition of the standard. Results show that both Tridosil and Azimex-500
contain sufficient amount of active ingredient which is required to achieve desired
therapeutic activity. It indicates that the samples of different brands are manufactured
according to GMP guidelines and have the quality which meets the standard
specifications.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) in East West University, Dhaka, Bangladesh.