In vitro sensitivity study of Zoxanide against clinical isolates of Entamoeba histolytica.

Abstract

Amebiasis is a very common disease allover the world. Almost 10% people of the world suffer from amoebiasis. Poverty, ignorance, overcrowding, poor sanitation and malnutrition favor transmission and increased disease burden. Entamoeba histolytica is the etiological agent of amoebic dysentery and amoebic liver abscess. The objective of this study was to find out the in vitro sensitivity of Nitazoxanide against clinical isolates of Entamoeba histolytica. Entamoeba histoiytica isolates were collected from the stool and then cultured in different culture medium. Zoxanide was prepared in different concentrations of 4.6, 2.3 ,1.15 , 0.575 , 0.287 ,0.143 and 0.07187 J.1M using the Dimethyl sulphoxide (DMSO) solvent. They were incubated for 24 hours at 37°C. After incubation the isolates of viable Entamoeba histoiytica were counted. The viable counts of the Entamoeba histolytica in each concentration of Zoxanide were compared to the initial concentration as well as the control. As the concentrations of Nitazoxanide decreases (4.6, 2.3, 1.15, 0.575, 0.287, 0.143 and 0.07187 flM), the inhibitory activity of Zoxanide was also found to be decreased. Further studies are needed to check the efficacy of other brands ofNitazoxanide.