Evaluation of In-Vitro Antimicrobial activity of Cissampelos pareira

Abstract

Cissampelos pareira (Menispermaceae family) is widely distributed in tropical regions of Asia, and used in the treatment of many diseases. The traditional use of this plant suggests its possible antibacterial properties. The present investigations evaluate the antimicrobial activity of Cissampelos pareira against selected gram positive, gram negative bacteria and fungal strains. In this study, the crude ethyl acetate, ethanol & water extract of Cissampelos pareira (stem, and leaves) was assed for their antimicrobial activity using disc diffusion method. The activity was performed against common pathogenic bacterial (Bacillus sereus, Bacillus subtilis, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi, Shigella dysenteriae, Shigella boydii, Vibrio mimicus, Candida albicans, Candida albicans, Saccharomyces cerevisiae,Vibrio parahemolyticus, & Bacillius megateri). The antimicrobial activity of the extract for the concentration of 400μg/disc and 800μg/disc were observed against the standard antimicrobial agent Cephradine. The activities were understood by observing and measuring the zone of inhibition for each concentration. Maximum activity was revealed by ethyl acetate than ethanol extracts but less active was revealed by water. This plant effective for treatment of different pathogenic diseases. The other parts of the plant like root, seed showed many activities that confirms the significance of the plant for medicinal compounds.