In vitro dissolution study to determine the Drug-Drug interaction of Atorvastatin Calcium-Metformin HCLMultivitamin Tablet

Abstract

The purpose of my research work was to determine the drug-drug interaction between Atorvastatin, Metformin HCL and Multivitamin by doing in vitro dissolution study. Because of drug-drug interaction dissolution rate can varies. To carry the study these drugs were collected from the market and drug-drug interaction study were determined. Dissolution is a test used to characterize the dissolution properties of the active drug, the active drug's release and the dissolution from a dosage formulation. Drug is administered orally in solid dosage forms, such as tablet or capsules, must dissolve in the contents of the gastrointestinal tract before drug absorption can occur. Often the rate of drug absorption is determined by the rate of drug dissolution from the dosage form. Therefore, if it is important to achieve high peak blood levels of a drug, it will usually be important to obtain rapid drug dissolution from the dosage form. So, dissolution rate per minute is very important for better absorption and for better therapeutic activity. To determine the drug-drug interaction and the differences in dissolution rate, Atorvastatin was given alone and it was given with Metformin and Multivitamin in a dissolution tester. This test was done on USPII Paddle apparatus, at 75rpm at 37degree Celsius. The dissolution medium was phosphate buffer at pH of 6.8 and it was analyzed by UV spectrometry at 242 nm. After dissolution test, the dissolution rate of Atorvastatin after 30 minute was 36.14% which is less than 50% and didn’t pass the BP specified dissolution test. But in the combination the dissolution rate was only 5.58%.Though the dissolution rate of Atorvastatin was less than 50%, but it was far more than the rate of combination. In case of combination they gave antagonistic activity and that’s why dissolution rate decreased.