In vitro antimicrobial susceptibility test of two Doxycycline (DOXACIL®& MEGADOX®) brands of Bangladesh

Abstract

Antibiotic resistance has become a major clinical and public health problem affecting both current and future generations. Doxycycline plays a vital role to protect the microorganisms. It acts against various types of bacteria. It’s clinical usefulness has been declining because of the appearance of an increasing number of doxycycline-resistant isolates of clinically important bacteria. The major objective of this study was to find out quality and safety of different brands of doxycycline available in Bangladesh. Two different brands (Doxacil® and Megadox®) doxycycline were collected from the local market of Bangladesh. In five different concentrations antibiotic test according to the disc diffusion method was used to determine zone of inhibition and this test was performed with four different microorganisms (Staphylococcus aureus, E.coli, Sreptococcus pneumonia, Haemophilus influenzae) for the two brands. Results obtained after performing the test, from the comparison with standard it was concluded that, all the brands have similar antimicrobial susceptibility like the standard. The variation found in the sensitivity pattern to these commonly used drugs in present study could be attributed to the prevailing usage and abuse of the drugs in the area under study. All the brands having values within acceptance range. Under antimicrobial susceptibility test, the two brands of doxycycline capsule and reference standard gave similar results against four microorganisms.