In vitro Antibacterial susceptibility test of Beuflox® (500 mg) and Deoflox® (500 mg) tablet against different bacterial isolates

Abstract

Ciprofloxacin is the most commonly used second generation fluoroquinolone antibiotic. It is a broad-spectrum antibiotic effective against both Gram-positive and Gram-negative organisms. It is also effective against H. influenzae, penicillinase-producing Salmonella typhi, Campylobacter spp. and Pseudomonas spp., N. gonorrhoeae. The objective of this study is to measure and compare the sensitivity of the selected antibiotics with the standard. It is significant to determine the quality of antibiotics because of rapid occurrence of antibiotic resistance due to use of substandard or poor quality drugs. The study of in vitro antimicrobial susceptibility test of Beuflox® and Deoflox® tablets were performed by using disc diffusion method. The clinical isolates of three different strains of E. coli and two other species of microorganisms (Salmonella Typhi and Pseudomonas spp.) were collected. The bacterial isolates were subcultured and disc diffusion test was performed where the discs with selected antibiotics were placed on the agar plate that had been inoculated with microorganisms and after overnight incubation the zone of inhibition was measured. The result of this study was shown that, zone of inhibition of E. coli isolates were collected from blood sample (13 mm for Beuflox® and Deoflox®) was resistance and urine sample (20 mm for Beuflox® and Deoflox®) was intermediate and stool sample (17 mm for Beuflox® and 12 mm for Deoflox®) was sensitive to Beuflox® and Deoflox® tablet by compared with the Ciprofloxacin standard. Beuflox® (40 mm) and Deoflox® (38 mm) tablets were also susceptible to Pseudomonas spp. isolates where the zone of inhibition of Ciprofloxacin standard was 40 mm. Salmonella typhi also susceptible to Beuflox® (39 mm) and Deoflox® (38 mm) tablets where the results were compared with the Ciprofloxacin standard (40 mm). Antimicrobial susceptibility test is essential for the selection of the antibiotic. The results of antimicrobial susceptibility testing should be combined with clinical information and experience when selecting most appropriate antibiotic therapy for the patient.