Pharmacological and Toxicological Study of Methanolic Extract of Curcuma Caesia with the Evaluation of Antioxidant and Cytotoxic Activity of Curcuma Caesia

Abstract

Purpose: The research work was carried out to determine the pharmacological activities and toxicology of methanolic extract of Curcuma Caesia. Evaluation of Antioxidant and Cytotoxic Activity of Curcuma Caesia. Method: Methanolic bark extract was administered orally to the animal model (Swiss albino) and the effects were determined by comparing with respect to control group which were treated with 5% CMC.. Different experiments were used to determine the pharmacological profile which was Collected from internationally published publications and journals. Result: The plant was found to have depressive and toxic effect. The CNS activity was evaluated by open field method. In the open field method and hole board experiment the crude extract of Curcuma Caesia (200mg/kg, 400mg/kg & 800mg/kg) dose dependently reduces the number of peripheral locomotion, central locomotion and leaning in the open field test and reduces the number of head dipping and head poking in the hole board test. The reduction is significant (***p<0.001) when it is compared to the standard drug. For finding chronic toxicity several tests are done such as CBC (Cell Blood count) test, Hepatic enzyme test and histopathological Studies. All data were analyzed by using SPSS analytical method. Conclusion: After summarize all the results it can say that bark of Curcuma Caesia may have several pharmacological activities. Curcuma Caesia was screened for their antimicrobial and antioxidant activities. The part which was used in the research study was methanolic extract of leaves. Methanolic extracts of Curcuma Caesia leaves exhibited strong antioxidant and antibacterial activities. First phyto-chemical test was done where methanolic extract of leaves showed high content of flavonoid & tannins. DPPH scavenging activity test & total phenolic content test is rendered then to monitor the anti-oxidant activity. In DPPH scavenging activity test The IC50 or inhibitory concentration of the sample was obtained ( 28.30 μg/ml ) much more higher than the standard ( Ascorbic acid ) compound. Methanolic extract of leaves also showed high phenoilic content ( 223.1333±24.5375 mg/g ).