Determination of CNS Depressant and Analgesic Activity of Bark of Artocarpus chama

Abstract

Phytochemical screening of Artocarpus chama revealed that the plant contains flavonoid, alkaloid. Flavonoid has anti-inflammatory and CNS depressant activity. The study was conducted to evaluate CNS depressant activity by open field and hole board test and analgesic activity by acetic acid induced writhing test and formalin induced pain method of bark of Artocarpus chama. In the study, it was found that the open field test of bark extract of methanol and pet ether has shown significant (p<0.001) decrease in locomotor activity in mice model compared to standard drug, diazepam. In case of Acetic acid induced writhing test, methanol and pet ether extracts at a dose of 400 mg/kg showed significant (p<0.001) decrease in writhing compared to standard drug, indomethacin. ACBM at a dose of 200 mg/kg and 400 mg/kg showed inhibition about 41.14% and 75.92% respectively. ACBP at a dose of 200 mg/kg and 400 mg/kg showed inhibition about 56.52% and 61.54% respectively. Formalin induced pain test, the bark extracts at a dose of 200 mg/kg and 400 mg/kg prevent the licking and biting activity of mice in a dose depending manner in the late phase compared to standard drug, ibuprofen. ACBM showed good inhibition about 42.32% in late phase at a dose of 200 mg/kg, ACBM at a dose of 400 mg/kg showed good inhibition about 58.36% in late phase. ACBP at a dose of 200 mg/kg and 400 mg/kg showed good inhibition in late phase about 66.04% and 51.19% respectively. It can be concluded that the plant might have CNS depressant activity and also analgesic activity.