Abstract:
Background: Entamoeba histolytica is the etiological agent of amoebic dysentery and
amoebic liver abscess. Amoebiasis is one of the most common health problems in the
developing countries. Almost 10% people of the world suffer from amoebiasi s. Poverty,
ignoranc, overcrowding, poor sanitation and malnutrition favor transmission and increased
disease burden. Objectives: To find out the in vitro sensitivity of Carica papaya seed extract against clinical
isolates of Entamoeba histolytica. Materials and methods: Entamoeba histolytica isolates collected from the stool and
cultured in different culture medium. Carica papaya seed extract prepared in different
concentrations of 0.12 mg/ml, 0.24 mg/ml, 0.49 mg/mI, 0.98 mg/ml, 1.97 mg/ml, 3.95
mg/ml and 7.98 mg/ml using the Di-methyl sulphoxide (DMSO) solvent and incubated at
24 hours and 48 hours respectively. After incubation the isolates of viable Entamoeba
histolytica were calculated.Result: Viable count of the Entamoeba histolytica in each concentration of Carica papaya
seed extract was compared to the initial concentration and with the control. After 24 hours
of incubation concentration of 7.89 mg/mI shows no presence of Entamoeba histolytica.
After 48 hours of incubation concentrations of 0.98 mg/mI, 1.97 mg/mI, 3.95 mg/mI, 7.89
mg/mI shows no presence of the clinical isolates of Entamoeba histolytica. Conclusion: The results show that the in vitro sensitivity of Carica papaya seed extract
against the Clinical isolates of Entamoeba histolytica.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) in East West University, Dhaka, Bangladesh.
